960化工网/ 文献/ 论文详情

Quantitative Analysis of Diubiquitin Isomers Using Ion Mobility Mass Spectrometry

ElizavetaI.Shestoperova,DaniilG.Ivanov,EricR.Strieter
ACS Environmental Au Pub Date : 04/04/2023 00:00:00 , DOI:10.1021/jasms.3c00016
Abstract
The diversity of ubiquitin modifications calls for methods to better characterize ubiquitin chain linkage, length, and morphology. Here, we use multiple linear regression analysis coupled with ion mobility mass spectrometry (IM-MS) to quantify the relative abundance of different ubiquitin dimer isomers. We demonstrate the utility and robustness of this approach by quantifying the relative abundance of different ubiquitin dimers in complex mixtures and comparing the results to the standard, bottom-up ubiquitin AQUA method. Our results provide a foundation for using multiple linear regression analysis and IM-MS to characterize more complex ubiquitin chain architectures.
引用文献
推荐文献
微信二维码
  • 微信公众号二维码
  • 关注官方微信公众号
  • 微信二维码
  • 微信扫码联系客服
平台客服