960化工网/ 文献/ 论文详情

A chimeric hairpin DNA aptamer-based biosensor for monitoring the therapeutic drug bevacizumab†

Mengyun Zhang,Feng Chen,Huihui Wu,Minyi Li,Jacques Crommen,Qiqin Wang,Zhengjin Jiang
Analyst Pub Date : 11/21/2023 00:00:00 , DOI:10.1039/D3AN01324C
Abstract

The accurate and rapid detection of specific antibodies in blood is very important for efficient diagnosis and precise treatment. Conventional methods often suffer from time-consuming operations and/or a narrow detection range. In this work, for the rapid determination of bevacizumab in plasma, a series of chimeric hairpin DNA aptamer-based probes were designed by the modification, labeling and theoretical computation of an original aptamer. Then, the dissociation constant of the modified hairpin DNA to bevacizumab was measured and screened using microscale thermophoresis. The best chimeric hairpin DNA aptamer-based probe was then selected, and a one-step platform for the rapid determination of bevacizumab was constructed. This strategy has the advantages of being simple, fast and label-free. Because of the design and screening of the hairpin DNA, as well as the optimization of the concentration and electrochemical parameters, a low detection limit of 0.37 pM (0.054 ng mL−1) with a wide linear range (1 pM–1 μM) was obtained. Finally, the rationally constructed biosensor was successfully applied to the determination of bevacizumab in spiked samples, and it showed good accuracy and precision. This method is expected to truly realize accurate and rapid detection of bevacizumab and provides a new idea for the precise treatment of diseases.

Graphical abstract: A chimeric hairpin DNA aptamer-based biosensor for monitoring the therapeutic drug bevacizumab
引用文献
推荐文献
微信二维码
  • 微信公众号二维码
  • 关注官方微信公众号
  • 微信二维码
  • 微信扫码联系客服
平台客服